Coding

Part:BBa_K5111006:Design

Designed by: Thiago Espurio Moraes   Group: iGEM24_City-of-London-UK   (2024-09-28)


FAD:protein FMN transferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 684
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 153
    Illegal NgoMIV site found at 205
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CDS naturally occurs on the minus strand. Reverse complement of sequence was used instead.


Source

ApbE CDS was taken from P.stutzeri (Clade I) ZoBell ATCC 14405.

References